Biotechnology - Sciene

PRODUCTION AND CHARACTERIZATION OF CHOLESTEROL OXIDASE PRODUCED FROM LOCALLY ISOLATED Pseudomonas aeruginosa H48.

Ninety one samples were collected from different clinical and environmental sources to isolate cholesterol oxidase producing Pseudomonas sp. , from these samples, 110 isolate were obtained, sixty of them were belong to Pseudomonas sp. Since they have the ability to grow on citrimide agar medium. Results of screening of these isolates for cholesterol oxidase production showed that Pseudomonas sp. H48 was the most efficient among the tested isolates in enzyme production since the activity of crude enzyme in culture filtrate was 1.71 U/ml.

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