Preliminary study on the tolerance of two wheat varieties of Triticum aestivum L. to the crude oil at the whole and cellular level
In an attempt to enhance crude oil tolerance in tissue cultures and intact plants of Triticum aestivum L., several experiments were carried out. Callus was induced using embryos isolated from the seeds of the two wheat varieties (Abu Ghraib-3 and Dijila) which were used as explants. Callus was induced and maintained on Murashige and Skoog, 1962 medium (MS) supplemented with (0.5) mg/l kinetin and (2) mg/l 2,4-D. Crude oil was added to the culture medium at different concentrations as a contaminating agent.