Biotechnology - Sciene

In an attempt to achieve optimum conditions for protoplast isolation, purification and fusion in two tomato (Lycopersicon esculentum) hybrids

This project was designed to induce somatic embryogenesis on different explants dissected from Solanum melongena L. plant. In this study, calli was induced on stem segments grown on MS (Murashige and Skoog, 1962)medium supplemented with NAA at 8mg/l and Kin at 0.1mg/l, Decreasing the supplemented agar to 5g/l increased calli induction significantly in the rate and time of induction. Calli initiated on stem segments was photographed under light microscope after one week on MS free of PGRs which shown embryos at globular stage. Decreasing the