Biotechnology - Sciene

In an attempt to treat infection of mice skin using ethanolic extract of  rosemary (Rosmarinus officinalis) callus cultures. Several experiments were carried out. Callus was induced on leaf explants and maintained for 30 days on Murashige and Skoog medium supplemented with 0.5 mg/l of benzyl adenine and 2.0 mg/l of 2,4-dichlorophenoxyacetic acid. Water and ethanolic extracts of R. officinalis leaves and callus cultures were investigated for their antimicrobial activity.

 A total of 110 samples were collected from different environments (waste waters, soil, and fresh waters) and locations in Baghdad governorate. Total isolates obtained from these samples were 85, 42 of them were suspected to be Pseudomonas spp. depending on their stability to grow on cetrimide agar.

The present study was designed to evaluate the immunological, cytogenetic and fertility effects of two extracts (methanol and hexane) of rosemary (Rosmarinus officinalis) leaves and stems in male albino mice. The immunological parameters included total and absolute counts of leucocytes and phagocytosis, while the cytogenetic evaluation was carried out through the detection of micronucleus formation in polychromatic erythrocytes and spermhead  bnormalities. The fertility assessment was considered in terms of sperm count and  otility.