In vivo and in vitro studies on the production of some secondary metabolites from Salvia officinalis L. and their antibacterial activity
In an attempt to increase the production of some secondary metabolites in tissue cultures of Salvia officinalis compared to the intact plant, several experiments were carried out. Callus was induced and maintained on MS medium supplemented with 0.5 mg/l kinetin and 0.05 mg/l 2,4-D from leaf and stem explants. NaCl was added to the culture medium at concentrations 50 or 100 mM as a stress agent for elicitation.