Biochemical study of Alcoholdehydrogenase produced from hydrocarbon utilizing pseudomonas Sp.
Number of efficient hydrocarbon utilizing bacteria of Pseudomonas spp. were tested for their ability to produce Alcoholdehydrogenase (ADH) enzyme. Pseudomonas sp. RB30 isolate was selected according to it's the highest ADH enzyme production, and this bacterium was identified as Pseudomonas fluorescens RB30. The enzyme was yielded the maximal volume activity of 0.34 U/ml when extracted by using lysozyme treatment as compared with low volume activity 0.19 U/ml resulted from sonication of the cells.
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