Purification and Characterization of α – Amylase Produced by The Local Isolate Xanthomonas campestris H6
The ability of Xanthomonas campestris strain H6 for the production of α – amylase was studied and it was found that Xc H6 is capable of producing the e xtracelluar α – amylase enzyme. Optimal conditions for the α – amylase production in PM1 medium were studied and the higher activity of this enzyme was obtained in PM1 medium supplemented with glucose 3.5 %, peptone 2 %, K2HPO4 2.5 % at 30 ºC and pH 7.0. Results obtained demonstrate that α – amylase produced by Xc H6 is a constitutive enzyme that is not subjected to ca