number:
1011
English
College:
department:
Degree:
Supervisor:
Dr. Abdul W. Baqir
year:
2005
- A total of (150) urine samples were collected from patient suffering from urinary tract infection (UTI) who referred to two hospitals in Baghdad (Al-Yarmoq and AL-Karama). Results declared that (116) of bacterial isolates were obtained from those patients after culturing on MacConkey agar and blood agar media for 24 hr at 37ºC. Identification showed that (16) of the isolates belonged to genus Proteus, which represent in percentage of isolation of (62.5%) to Proteus mirabilis and (37.5%) to Proteus vulgaris.
- Another part of the study includes isolation of Lactobacillus (LAB) isolates for using as probiotics to test its ability to inhibit growth and adhesion of Proteus mirabilis. Ten isolates of LAB were obtained from (15) yoghurt samples were collected from local Baghdad City Markets after culturing on DeMan Rogoza Sharpe (MRS) medium for 24 hr at 37ºC.
- When the isolates of Proteus mirabilis were subjected to the sensitivity test against (12) antibiotics, result showed that amikacin, ciprofloxacin and gentamycin were the most effective isolates when bacterial sensitivity to them were in percentages of 90, 80 and 70%, respectively. Penicillin, amoxicillin and tetracycline, on the other hand, were the least effective antibiotics when bacterial sensitivity to them was not exceeded 10, 20 and 20% respectively.
- Upon antibiotics sensitivity results, firstly, one isolate (P. m. 9) of Proteus mirabilis was chosen due to its resistance to all (except amikacin) antibiotics, and secondly, two isolates of LAB; Lactobacillus plantarum (Lb. p. 3) and Lactobacillus acidophilus (Lb. a. 11) due to their highest inhibitory activities, were selected to be used in further experiments.
- Minimum inhibitory concentrations (MICs) were determined for the three-fold concentrated filtrates of LAB against adhesion property of Proteus mirabilis (P. m. 9). Results showed that filtrates were able to lowering adhesion of pathogenic bacteria to the epithelial cells when the average decreased to only (3-10) bacteria / cell instead of (44-55) bacterial / cell for the control treatment.
