M.Sc.

Mutations in NPM1 gene ranked within the most frequent known genetic marker in AML. In order to detect the frequency of NPM1 mutations within the region of exon 2 and exon 3 in Iraqi AML patients using conventional polymerized chain reaction technique, the region from 2380 bp to 4140 bp of NPM1 gene,which represents the region flanking both exon 2 and 3, was amplified by using two specific primers in a thermal cycler. Results showed that the concentration of genomic DNA after extraction was ranged between (52-93) µg/ml with purity of (1.8-1.92).

   This study aimed to synthesise carboxymethylnanochitosn as nanochitosan derivative from nanochitosan synthesized from soluble chitosan (low molecular weight) and encapsulate streptomycin then characterize and bioassay them on pathogenic gram positive and gram negative bacteria and Rhabdomyosarcoma cell- line. Ionic gelation method was used to synthesize nanchitosan from soluble and insoluble chitosan (high molecular weight). Dissolved chitosan was found to be superior to non-soluble in the preparation of nanochitosan.

     The present study was designed to detect the effect of Saussurea lappa and Cyperus esculentus extracts on male albino mice fertility in addition to the histopathological changes in liver, kidney and testes. Saussurea lappa extract was prepared by maceration of 50 gm of Saussurea lappa roots with 90% methanol, then chemical detection of flavonoids, alkaloids, tannins, saponins, terpenes and steroids was carried out.