M.Sc.

Macrophage Migration Inhibitory Factor Gene Polymorphism and some Immunological Aspects of Urothelial Carcinoma Patients

 This study was designed to shed light on the association between polymorphism of macrophage migration inhibitory factor (MIF) gene at -173 locus as risk factor of bladder cancer in Iraqi population by PCR-RFLP ,estimation the serum level of MIF with some cytokines (IL-6, IL-10 and TNF-α) by sandwich ELISA and  determine the expression of several marker (CD74, CD44, P 53  and P 21 ) by  immunohistochemical staining.

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Transformation of Lactobacillus acidophilus with Hygromycin Gene Originated from Streptomyces hygroscopicus

           To Investigate the ability of Streptomyces isolate to produce hygromycin B antibiotic as antithelmantic agent in poultry feed and selecting a suitable expression vector encoded hygromycin B phosphotransferase resistance gene and transforming Lactobacillus isolateby electroporation , from a total of 53 Streptomyces isolates, DNA was extracted and screened for the presence of hygomycin B gene using specific designed primer sets in Polymerase Chain Reaction (PCR) technique.  Only three of the isolates were positive for the existence of such a gene which

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Preparation and Characterization of Doxorubicin Loaded Tri-Block Copolymer (PCL-PEG-PCL) and its Cytotoxic Effect on Breast Cancer Cell Line MCF-7

The present study aimed to prepare, characterize and explore the cytotoxicity effect of doxorubicin (Dox) loaded biodegradable amphiphilic triblock copolymer poly caprolactone (PCL) poly ethylene glycol (PEG) poly caprolactone  (PCL-PEG-PCL) micelles against breast cancer cell line MCF-7.The physicochemical properties and in vitro test including cytotoxicity of the micelles were examined.

English