M.Sc.

Antimicrobial activity and cytotoxicity of active compounds of Withania somnifera extracts

       In this study Withania somnifera leaves were collected from gardens of Al-Nahrain University. Then the leaves powder was used to get three type of extracts, each type of Withania extracts (water, ethanol and acetone extract) were subjected to chemical analysis to identify the active compounds in each extract which indicated that alkaloids, glycoside, saponins, flavonoids, fixed oil were present in ethanol, acetone and water extracts, while absent of terpene in water extract and protein in acetone extract, Tannins were not found in all extract types.

English

Osteoblast formation from MesenchymalStem Cells Derived from mouse Bone Marrow in vitro

This study was designed to evaluate osteogenic potential of mesenchymal stem cells (MSCs) isolated from mouse bone marrow in vitro. MSCs were isolated by collecting the thigh bone of 50 male albino mice, both the femur and tibia were collected and cells were flushed from bones and MSCs isolated based on the ability of adherence to plastic surfaces. Reactivity MSCs to CD105 and CD34 were tested by immunocytochemistry.

English

Production, Characterization and Immobilization of Laccase Enzyme from Bacillus cereus Local Isolate

In this study, fifty five soil samples were collected from different locations in Baghdad city. Many Gram positive and negative bacterial isolates were obtained;of which 39 were identified as Bacillus spp. upon subjecting to morphological and microscopic tests. Laccase enzyme activity was determined by quantitative methods using a syringaldazine as substrate for growth of these isolates. Results indicated that 17 of these isolates were laccase producer with different specific activity ranged between 98-600U/mg  protein.

English