Biotechnology - Sciene

The objective of this work was the detection of DNA polymorphisms among seven salt-tolerant bread wheat {Triticum aestivum L.) lines provided by the Iraqi Atomic Energy Organization, and one variety (Maxipac) known to be salt-sensitive as a control. This was achieved by the of Random Amplified Polymorphic DNA (RAPD) markers. DNA polymorphisms obtained using RAPD analysis were used to tentatively identify all samples tested, and to determine the genetic distance (GD) and the genetic relatedness among them.

Some of the enzymes of the wild type Micrococcus sp. strain Gl were detected & it was found that strain Gl was positive for catalase <§c nitratase, then depending on a comparison of intracellular activities for each of catalase & protease enzyme in different NaCl concentrations (2, 10 & 20 %), catalase was choosed for the present study according to its importance & its highest production in all NaCl concentrations. However maximal function of catalase obtained from cells grown in 10 % NaCl as compared with catalase produced from 2 % & 20% NaCl grown cells.

Study was conducted for cytogenetic analysis of S U.99 cell line, for 6 successive passage in tissue culture. Results were compared with normal cytogenetic analysis of 15 normal female BALB/c mouse of spleen cells classified into of 3 groups.