Preparation and evaluation of coombs test and spt for iso-antibodies and anti-Rh (D) antibodies diagnosis .
Abstract: Anti-globulin reagent is the most important tool used in immunohematology laboratories and blood banks for allo-antibodies screening during incompatible pregnancy and cross matching. this study, attempts were made to prepare a suitables monospecific reagent, for Rh-D allo-immunization during pregnancy; by immunizing rubbits with fresh human serum and purified human immunoglobulin (Ig) anti-human immunoglobulin and anti-human serum were produced and used at a dilutions 1/120 and 1/100 , respectively.