Biotechnology - Sciene

In this study, twenty male BALB/c mice were fed on lead oxide containing diet at 50 part per million (ppm) for four months. Tail biopsies were used for DNA isolation as normal genomic DNA before lead exposure to be compared with genomic DNA from liver and kidney after the exposure. Blood lead concentrations were measured using atomic-spectroscopy. Blood also drawn from each animal to examine phagocytic activity of peripheral blood leucocytes to engulf Candida albicans and results were compared with control group.

Number of efficient hydrocarbon utilizing bacteria of Pseudomonas spp. were tested for their ability to produce Alcoholdehydrogenase (ADH) enzyme. Pseudomonas sp. RB30 isolate was selected according to it's the highest ADH enzyme production, and this bacterium was identified as Pseudomonas fluorescens RB30. The enzyme was yielded the maximal volume activity of 0.34 U/ml when extracted by using lysozyme treatment as compared with low volume activity 0.19 U/ml resulted from sonication of the cells.