Determination of Ampicillin Trihydrate Alone and in Presence of Some Metals By Derivative Spectrophotometric Method
Refluxed ampicillin trihydrate was determined using conventional and derivative UV absorption spectrophotomerty.The calibration curve for refluxed ampicillin trihydrate at 339 nm was found to have a linear range extended from 4 to 200 ppm with relative standard deviation for the slope of 0.0082 and correlation coefficient of 0.9999 with detection limit of 0.5 ppm. The effect of pH on the determination of ampicillin trihydrate at this wavelength was investigated.