Biotechnology - Sciene

Aqueous and methanolic extracts of dried aerial parts of Hibiscus sabdariffa and Harpagophytum procumbens were tested for their antimicrobial and curing activity against two microorganisms Escherichia coli HB 101 and Pseudomonas aeruginosa RB19 Chemical investigation on these extracts showed that Hibiscus sabdariffa methanolic extracts contain flavonoids and water extract contain glycosides and Saponins while the Harpagophytum procumbens methanolic extracts contain flavonoids and water extract contain glycosides. .

This project was conducted to evaluate the activity of S-layer proteins isolated from Lactobacillus in comparison with the activity of concentrated filtrate of Lactobacillus against some pathogenic microorganisms and against tumor cell lines in vitro. Twelve isolates of Lactobacillus spp.

This study aimed to modify Staphylococcus aureus by using physical and chemical mutagens and subjections the cell wall to the hydrolytic activity of lysozyme. Chemical mutagenesis by N-methyle-N-nitro-N-nitroguanidine, was achieved by incubating the cell suspension of Staphylococcus aureus with 100μg/ml MNNG for different periods of time (5, 10, 15, 20, 25 and 30min).Aliquot of 100μl of the bacterial suspension subjected to the lethal and mutagenic effect of MNNG, then spread on BHI agar and incubated at 37°C over night in dark.