Biotechnology - Sciene

Using Bean pod mottle virus (BPMV) as virus-induced gene silencing (VIGS) vector to target multiple genes in Glycine max (L.) Merr.

Plant viral vectors are valuable tools for host gene analysis, thus possessing important applications as reverse genetics tools for gene function studies. Bean pod mottle virus (BPMV) is a bipartite, positive-sense (+) RNA virus of Secoviridae. Insertion of relatively short cDNA fragments (96nts) corresponding to different regions of soybean (Glycine max) phytoene desaturase1 (GmPDS1) into the 3' UTR of BPMV RNA2 initiated weak photobleaching manifested by white spots along the veins of soybean leaves.

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