In this study Withania somnifera leaves were collected from gardens of Al-Nahrain University. Then the leaves powder was used to get three type of extracts, each type of Withania extracts (water, ethanol and acetone extract) were subjected to chemical analysis to identify the active compounds in each extract which indicated that alkaloids, glycoside, saponins, flavonoids, fixed oil were present in ethanol, acetone and water extracts, while absent of terpene in water extract and protein in acetone extract, Tannins were not found in all extract types.