Biotechnology - Sciene

           To Investigate the ability of Streptomyces isolate to produce hygromycin B antibiotic as antithelmantic agent in poultry feed and selecting a suitable expression vector encoded hygromycin B phosphotransferase resistance gene and transforming Lactobacillus isolateby electroporation , from a total of 53 Streptomyces isolates, DNA was extracted and screened for the presence of hygomycin B gene using specific designed primer sets in Polymerase Chain Reaction (PCR) technique.  Only three of the isolates were positive for the existence of such a gene which

The present study aimed to prepare, characterize and explore the cytotoxicity effect of doxorubicin (Dox) loaded biodegradable amphiphilic triblock copolymer poly caprolactone (PCL) poly ethylene glycol (PEG) poly caprolactone  (PCL-PEG-PCL) micelles against breast cancer cell line MCF-7.The physicochemical properties and in vitro test including cytotoxicity of the micelles were examined.

Mutations in NPM1 gene ranked within the most frequent known genetic marker in AML. In order to detect the frequency of NPM1 mutations within the region of exon 2 and exon 3 in Iraqi AML patients using conventional polymerized chain reaction technique, the region from 2380 bp to 4140 bp of NPM1 gene,which represents the region flanking both exon 2 and 3, was amplified by using two specific primers in a thermal cycler. Results showed that the concentration of genomic DNA after extraction was ranged between (52-93) µg/ml with purity of (1.8-1.92).