M.Sc.

The main aim of this study was obtaining efficient bacteria capable of desulfurizing and/or utilizing organosulfur compounds that found in petroleum and its derivatives. For this purpose oil contaminated soil samples with a history of oil pollution were collected from forty different sites in Iraq.Sixty three bacterial isolates were obtained and these isolates capable to utilize dibenzothiophene (DBT). Results showed that most of these isolates were capable to grow on DBT in the presence or without glycerol (carbon source).

     In order to isolate Streptococcus mutans, 80 swab samples were collected from dental caries of patients attended Al-Dora Health Center and Al-Zewiya Health Center in Baghdad city. From these samples, 98 bacterial isolates were obtained. Results of  identification depending on morphological, cultural and biochemical tests showed that 10 of these isolates were belonged to Streptococcus mutans. The results of identification were confirmed by using VITEK-Π system. All isolates were screened to examine their ability to mutacin production.

 In an attempt to treat infection of mice skin using ethanolic extract of rosemary (Rosmarinus officinalis) callus cultures. Several experiments were carried out. Callus was induced on leaf explants and maintained for 30 days on Murashige and Skoog medium supplemented with 0.5 mg/l of benzyl adenine and 2.0 mg/l of 2,4-dichlorophenoxyacetic acid.  Water and ethanolic extracts of R. officinalis leaves and callus cultures were investigated for their antimicrobial activity.