K. pneumoniae isolates was diagnosed molecularly by using polymerase chain reaction, 16S rRNA, rmpA and kfu genes were amplified and study the prevalence of the K serotypes, as well as, genes (except kfu gene) were amplified by using multiplex PCR. Results showed that all the isolates of K. pneumoniae gave a clear band with a molecular size 130 bp when PCR was performed with the primer that target the 16S rRNA.
Ficus religiosa plant is an important medical plant and traditionally used to treat various diseases including mastitis, otitis media, pharyngolaryngitis, urethritis, dysmenorrhea and diabetis.
Plant viral vectors are valuable tools for host gene analysis, thus possessing important applications as reverse genetics tools for gene function studies. Bean pod mottle virus (BPMV) is a bipartite, positive-sense (+) RNA virus of Secoviridae. Insertion of relatively short cDNA fragments (96nts) corresponding to different regions of soybean (Glycine max) phytoene desaturase1 (GmPDS1) into the 3' UTR of BPMV RNA2 initiated weak photobleaching manifested by white spots along the veins of soybean leaves.
