Cytological effects of Nigella sativa (black seed) callus extract on mouse bone marrow cells
The aim of this study was to initiate callus from black seed Nigella sativa to extract thymol substance and then study its potency to inhibit the cytogenetic effects of mitomycin-C (MMC) on laboratory mice (in vivo). Establishment of Nigella sativa (black seed) seedlings in tissue culture was achieved in our laboratory by using Murashige and Skoog medium, (1962) (MS) and optimization of cultural medium using a combination of 2,4-D and Kin concentrations The seeds were cultured in sterilized distilled water (after their steriliza