Biotechnology - Sciene

          The present study was conducted to evaluate some cytogenetic and immunological parameters in Down syndrome (DS) children and their parents. Ten families with children of DS were selected; in addition five families with normal children were included as a control group. The cytogenetic parameters; blastogenic index (BI), mitotic index (MI), chromosomal aberrations (CA), micronucleus (MN), sister chromatid exchange (SCE) and replicative index (RI) were assessed in lymphocytes of cultured peripheral blood.

        A total of 37 samples were collected from different clinical cases (ear, blood, sputum, burns, cerebrospinal fluid and cystic fibrosis samples) and environments (water and soil samples)from different locations in Baghdad governorate. The total isolates obtained from these samples were 57 isolates,13 of them were identified as Pseudomonas spp. after detecting it's ability to grow on cetrimide agar. Biochemical tests were carried out  on these 13 isolates.

 Six to eight weeks old white mice sharing the same mother and father were injected intraperitoneally with different dosages 0.1, 0.2 and 0.5mg/kg body weight (bw) of the chemotherapeutic antitumor drug Mitomycin C (MMC). The effect of the drug on mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) was investigated by molecular techniques and cytogenetic analyses were performed on spleen and bone marrow cells.