Biotechnology - Sciene

Purification and Characterization of α – Amylase Produced by The Local Isolate Xanthomonas campestris H6

    The ability of Xanthomonas campestris strain H6 for the production of α – amylase  was studied and it was found that Xc H6 is capable of producing the e xtracelluar α – amylase enzyme.  Optimal conditions for the α – amylase production in PM1 medium were studied and the higher activity of this enzyme was obtained in PM1 medium  supplemented with  glucose 3.5 %, peptone 2 %, K2HPO4 2.5 %    at 30 ºC and pH 7.0.   Results obtained demonstrate that α – amylase  produced by Xc H6 is a constitutive enzyme that is not subjected to ca

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