Effect of physical and chemical mutagens on Staphylococcus aureus cell wall
This study aimed to modify Staphylococcus aureus by using physical and chemical mutagens and subjections the cell wall to the hydrolytic activity of lysozyme. Chemical mutagenesis by N-methyle-N-nitro-N-nitroguanidine, was achieved by incubating the cell suspension of Staphylococcus aureus with 100μg/ml MNNG for different periods of time (5, 10, 15, 20, 25 and 30min).Aliquot of 100μl of the bacterial suspension subjected to the lethal and mutagenic effect of MNNG, then spread on BHI agar and incubated at 37°C over night in dark.