In an attempt to treat infection of rabbit eyes using water and ethanolic extracts of chamomile (M. chamomilla) flowers compared to callus cultures extracts. Several experiments were carried out. Callus was induced and maintained on MS medium supplemented with 3.0 mg/l BA and 0.1 mg/l NAA initiated from seedlings. Plantlets regenerated on the same hormone combination after 40 days in culture. Water and ethanolic extracts of M. chamomilla flowers and callus cultures were investigated for their antimicrobial activity. It contained resins, tannins, coumarines, phenols, flavonoids and very little amounts of glycosides. Chamomile flowers water extract showed better inhibitory effect than ethanolic one against Staphylococcus aureus and Bacillus ceries and to a lesser extent to Pseudomonas aeriginosa and Escherichia coli. Callus extracts showed an inhibitory effect on the same bacterial isolates than flower extracts particularly on growth of S. aureus and B. ceries. The Minimum inhibitory concentration (MIC) of the flower extracts showed that water extract had better activity than ethanolic one. It has been noticed that all extracts of have a anti inflammatory effect at a concentration (40mg/ml). However, these extracts showed a antibacterial activity at a concentration 40mg/ml that applied on laboratory rabbit eyes.