Preparing and Studying Nano Silver Particles and Their Anti Bacterial Effect

The target of this research is to prepare nano silver particles by laser ablation in both deionized and magnetized water. The properties of nano silver particles and their bacterial effects with different parameters’ were studied. The magnetic water was laboratory preparing by square magnets with magnetic flux of 200 mT applied on the deionized water which pass through it 10ns Q.swiched Nd-YAG  laser of wave length 1064nm with different energies and different laser pulses number were used with and without applied electric field. Optical properties for the nano silver colloidal by UV-Vis technique was investigate. The absorption for the silver spectrum was found lied at 420-430nm, i.e. involved visible region. The absorption peaks have different height due to different both the shape and concentration of nano silver in the colloidal.TEM technique was used for investigating both size and shape of nano silver particles. The nano silver particles have different shapes such spherical, spindles, etc…. depending upon two parameters is laser energy and laser pulses number. The effect of nano silver particles on bacteria growth of three types of bacteria (staph, ps., E.coli) were studied in details under effect of applied electric field of 3000V/m. There is no inhibition zone was obtained when used different silver colloidal except the colloidal prepared under 600mJ and 1000laser pulses, where this colloidal gives an effected property and ability to determine the bacteria growth type ps. In the range of inhibition of 25nm.The results of the TEM show that the shape of nanoparticles affecting the growth of bacteria was spherical and its size ranges between32-50nm.