Molecular study of Thrombosis in a Sample of Iraqi Patients

This study aimed to investigate the association of F5 gene single nucleotide polymorphism (SNP) with the incidence of thrombosis. Blood samples werecollected from04 patientsduring the period from November 2014 to January 2015, from critical care unit (C.C.U) of (Yarmook Hospital, Kadhimiya  Hospital),as well as from 10unrelated healthy control group. This study found the age group between 50 to 60 are more susceptible to thrombosis 45% and the thrombosis was more frequent in male 55% from female 45% the significant (p<0.01). Deoxyribonucleic acid (DNA) was extracted from whole blood samples,whereas, serum samples were analyzed using troponin test (TNT) for detection of thrombosis. Polymerase chain reaction (PCR) was achieved on extracted DNAusing eleven specific primers for F5 gene:the first primer (Fve3) with product size (228bp),second primer (Fve4) with product size (310bp), third primer (Fve6)with product size(547bp),fourthprimer (Fve7) with product size (241bp),fifth primer (Fve8)with product size(306bp),sixth primer (Fve12) with product size(286bp), seventh primer (Fve13a)with product length (260bp) ,eighth primer (Fve13c) with product size (317bp) ,ninthprimer (Fve15) with product size (600bp) ,tenth primer (Fve16)with product size (333bp) and eleventh primer (Fve25)with product size(390bp).PCR products of F5 gene were sequenced. Result found to be change in DNA which where mostly SNP. This change was in three types: substitution 24.86%,insertion 28.57% and deletion 28.57%, a Leiden mutation was also identified among patients.