Biochemical and Histopathollogical Study of Moringa oleifera Extract on the Fertility in Male Mice

This study was designed to find out the effect of Moringa oleifera leaf extracts on fertility of male albino mice. M. oleifera  leaf extract was prepared by maceration of 50 gm of M. oleifera leaves with 70%
methanol, then chemical detection of flavonoids, alkaloids, tannins, saponins, glycosides, terpenes and steroids were detected. Isolation of Flavonoid from these extracts. The use of thin layer hromatography
technique helped in detection of Qurciten, Rutin and Luteoline of flavonoids extract; the presence of qurciten and rutin confirred by high performance liquid chromatography technique.  Total flavonoid of M.
oleifera leaf extracts was calculated by  Spectrophotometric technique. The 30 mice were divided equally into six groups,Group1:(negative control): mice treated with water, Group2 (positive control): mice treated with 0.36 mg/kg of Mesterolone (Proviron), Group3 (positive control): mice treated with 0.5 mg/kg of Ginseng drug (ginsavet) Group4: mice treated with 2.5 mg/ ml of extract (100mg/kg), Group 5: mice treated with 5 mg/ ml of extract (200 mg/kg), Group6:mice treated with 7.5 mg/ml of extract (300 mg/kg). The extract was administered orally for 15 days.The effect of methanolic extract on the sperm including sperms
concentration, percentage of viable sperms, percentage of morphologically abnormal sperms and an assay of serum testosterone were studied,.Then the histopathological sections of liver, kidney and testis were examined. The results showed  a significant increase (p ≤ 0.05) in sperm concentration after 15 days of treatment with plant extract at doses 100 and 200mg/kg when compared with controls and with other grouptreatment at dose 300 mg/kg. A significant increase (p ≤ 0.05) in dead sperm was observed aftertreatment with plant extract at doses 300 mg/kg when compared withcontrols and with other treatments, (extract doses of 100 and 200mg/kg). A significant increase (p ≤ 0.05) Serum testosterone in micetreated with 100 and 200 mg/kg when compared with controls and withother group treated with plant extract at dose of 300 mg/kg. Thepathological study of testis, Liver and kidney in controls and in mice treated with plant extract at doses 100 and 200 mg/kg show no significantchanges, while at dose 300 mg/kg showing the presence of negativechanges.