This study was constructed to discuss an issue regarding infertility cases that was attributed to hyperprolactinemia. About 150 blood samples were collected from women suffering hyperprolactinemia and 50 from normal subjects served as control for comparison. Test subjects were divided into three age groups: 20-30 years old, 31-40 years old and 41-50 years old. Tests of fertility hormones luteinizing hormone, follicle stimulating hormone and prolactin were performed for all study groups. It was found that there was a significant P<0.05 difference in hormone concentration for patients when compared with control. Luteinizing and Follicle stimulating hormone recorded a significant decrease while prolactin recorded a significant increase when compared with control. RNA isolation from serum was possible due to high expression of prolactin gene in study groups since an average concentration of 300 ng was obtained from serum. At molecular level analysis using three specific primers designed for this study, showed that there is an aberration at the expression level of RNA in some of hyperprolactinemia patients while prolactin receptors were normal. It was concluded that in all patients feedback inhibition mechanism that controls prolactin level, was disrupted in addition some of patients studied were candidates for breast cancer as was reviewed from family history and most of hyperprolactinemia cases for subjects studied were attributed to hyper expression of prolactin gene. Protein analysis of blood from patients showed that a significant increase in albumin, this was regarded to increased level of prolactin in the blood and this protein function as a carrier for hormones.
