Cytotoxic Study Of Artemisia herba-alba Extraction On S.U.99 Plasmacytoma Cell Line And Some Aspects Of Mice Immune Response

  This study was designed to determine the biological effect of diethyl ether extract of Artemisia herba-alba on mice immune response, normal and tumor cell line and visceral organs. A total of 112 male BALB/c mice have been used in this study.  In the in vivo study, 92 mice have been used. In lymphocyte transformation assays spleen single cell suspensions have been prepared as well
as peripheral blood samples have been taken to examine the effect of extract on lymphocyte transformation. The results were showing a significant increasing in the percentage values of blastogenic response in comparision with control 1 and control 2 (b<0.05). While the results of phagocytosis assay showed a significant increasing in the percentage values of phagocytic cells in comparision with
control 1 and control 2.  The cytogenetic assay was done by using spleen and blood lymphocytes as well as testis to study the effect of extract on cells mitosis. The results of this test were indicating a significant increasing in mitotic index values when compared with control 1 and control 2 (b<0.05).   pleen, kidney, liver and testis samples have been prepared for histological studies and the results cleared that there was no changes in the level of tissues and no extract precipitation have been observed.  In the in vitro study. A total of 20 mice have been used. In this study three tests have been done. The first test on studying the effect of extract on lymphocyte transformation. The results showed a significant increasing in the percentage values of blastogenic response in both blood and spleen lymphocytescompared with control 1 and control 2 (b<0.05).The second test was phagocytosis assays. The results also revealed an increasein the percentage values of phagocytic cells in compared with control 1 and control 2 (b<0.05). While the third test was cytogenetic study of the effect of extract on spleen and blood lymphocytes mitosis. The results were showing a significant increasing in the percentage of mitosis index values in compared with control 1 and control 2 (b<0.05). The finale experiment was applied to study the cytotoxic effect of extract on both normal and tumor cell line. The results of the cytotoxic effect of extract on tumor cell line showed growth inhibition of cells in different times of incubation with extract (24, 48, 72 hr. and 4 days) in compared with control 1 and control 2. The results of cytotoxic effect of extract on normal cells pointed a growth stimulation of cells in the last two concentrations (60 and 45 mg/well), while no effect was detected on cells growth by other concentrations compared with control 1 and control 2.