Studies concerning the effects of diclofenac on the renal function were published. Since no information on its effect on the succinate dehydrogenase enzyme (which play an important role in the renal transport activity) have taken by consideration, which was the goal of this study. In this study (12) healthy adult male mice were used, (6) mice were injected intramuscularly with 0.5 ml diclofenac sodium (0.05 mg/kg) one time in the day for 7 days, and the remained considered as control group. After one week all animal groups were sacrificed by spinal dislocation. Sample from right kidney were obtained and preceded for light microscopic and electron microscopic examination.
The results have shown the following:
1. A significant decrease (p > 0.05) in the body weight as well as in organ/body weight ratio was observed.
2. Light microscopic examinations have shown many alterations in both renal corpuscles and kidney tubule, which include: necrosis of several renal tubules with the formation of large spaces in the interstitium component of some region of kidney structure. As to the glomeruli, a focal segmental glomerulosclerosis with collapse of capillary loops was observed.
3. Electron microscopic examination have shown a spectrum of changes in kidney tubule from apparently normal to Franz-necrosis, which reflect a state of acute renal failure.On the other hand renal corpuscles have shown a variable changes in renal corpuscle cells, represented with irregularities and increment of basement membrane thickness, proliferation of mesangial cells, and degeneration of podocytes.
4. The ultrahistochemical study of succinic dehydrogenase enzyme (SDH) reflect an increament of the enzyme activity in the proximal and distal convoluted tubule of animal treated with diclofenac.
From this study it has been concluded that:
- Diclofenac treatment caused acute renal failure.
- Treatment with diclofenac caused a focal segmental glomerulosclorosis with collapse of capillary loops.
- Treatment with diclofenac has a marked effect on (SDH) activity, which plays an important role in transport activity of renal tubule.
