The study included estimating the synergetic effect of Chamomilla recutita callus and flower extracts and surlactin produced by Lactobacillus acidophilus on some bacterial types causing eye infections as follows: To study the effect of C. recutita extracts, callus was induced from chamomile seeds, and it was maintained on Murashige and Skoog, 1962 (MS) culture medium supplemented with 1.0mg/l Benzyl Adenin (BA) and 0.1mg/l 2,4-dichlorophynoxyacetic acid (2,4-D). Results showed that flower (water and ethanolic) extracts have antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus which cause eye infections. The chemical constituents of these extracts were detected. Results revealed that they contain tannins, phenols, coumarins, flavonoids, resins and glycosides. The results also showed that ethanolic extract of flowers has higher antibacterial activity than water extract against P. aeruginosa and S. aureus. Callus extracts (ethanolic and water) showed higher antibacterial activity against the same bacterial types. Minimum inhibitory concentration (MIC) was determined for the extracts against the tested bacteria. Results showed that ethanolic extract has higher activity than water extract and the results varied according to the bacterial types. Twenty five vaginal swabs from outpatients’ healthy women were collected from Kamal Al-Samarai hospital, Baghdad to isolate and identify L. acidophilus from April 2007 to December 2007. Three of the isolates were diagnosed as L. acidophilus which represented 20% of the total number of bacterial isolates, other Lactobacillus types represented 65% (20 isolates), while other bacterial types represented 15%. The ability of L. acidophilus to produce surlactin was detected after measuring its biological activity to inhibit the adhesion of biofilm formed by bacteria (P. aeruginosa) to surfaces using test tube method.
