Abstract: The photobiostimulation is one of the interesting laser applications in medicine. Practically in Iraq there is no real attention paid to these medical applications of laser and there is no much knowledge about the benefits of the laser biostimulation of skin and tissue burn healing. This work focused on studying the effect of diode laser (637-785nm) with two different powers on the burn healing. The study is based on using 40mature, male albino mice divided into five groups, each of eight animals and all groups were burned using an electrical soldering unit on the back of each mouse for five seconds to create a 3rd degree burn of 1 cm in length. Four groups were treated with diode laser, the first group (S1) is treated with 785nm, 3.5mW CW diode laser for 3 minutes to get energy density = 3J/cm2,the second group (S2) is illuminated by using CW diode laser of 637nm, 2mW for 5 minutes with the same energy density. While the pulsed groups is treated by pulsed diode laser (637nm) of pulse repetition frequency (p.r.f) 1 KHz and two different duty cycles, 50% duty cycle for group(S3) and 25% for group (S4). One group of these animals was not irradiated by laser energy; it is used as a control group. The animals were irradiated three times per week. The first laser irradiation was given immediately after burn creation followed by the two other exposures at the next 2nd and 3rd day after burning.