Production and evaluation of diagnostic reagents for hereditary plasma clotting factor disorders a clinical survey.

The diagnosis of hereditary plasma clotting factor disorders is critically dependent on laboratory procedures.
Because of the blockade, most of the reagents that are needed for performing the tests are not available. In this study, attempts were made to prepare a collection of biological reagents that can be used to make a full diagnosis of the disorders. An uncomplicated and inexpensive procedures were chosen to prepare the reagents. For the PT test, two different reagents were prepared, the first one is a reagent prepared from human placenta, it is the first preparation in our country that utilize human placenta! tissue for the preparation of this reagent. The other reagent was prepared from rabbit brain tissue. For the APTT test, three preparations were made, one from bovine brain and the two others from soybean, those sources are also first to be used in our country for the preparation of APTT reagent. Another reagent was prepared for the TT test from citrated bank plasma. Aluminum hydroxide gel have been prepared to utilize it in the preparation of adsorbed plasma, in addition, serum reagent, aged plasma, and adsorbed serum were prepared. Those reagents are used in the differential screening test. Factor n deficient plasma, and factor V deficient plasma were prepared from human plasma, factor VH and X deficient plasma was prepared from bovine plasma, and antibodies raised against vWF:Ag were prepared by immunizing rabbits with Factor Vm concentrates. Using these reagents together with the available commercial reagents, a survey was made to determine the diversity of the defects in our patients, the number of affected patients, the geografical distribution of the affectted families, the incidence of viral infections, the severity of the defects, the mortality rate, and the family history of the affected persons.