Synthesis and Cytotoxic Activity of Some Pyrimidine Derivatives

This research is divided into two parts: Part one: This part involved in the synthesis of some pyrimidine derivativeswhich play an important role in the medicinal chemistry because they possess promising cytotoxic activity. The synthesized compounds: 1. 5,6-diaminouracil-2,4-diol hydrochloride. 2.5,6-diamino- 2-mercaptopyrimidine-4-ol hydrochloride. 3.2-mercapto-6-methylpyrimidin-4(3H)-one. 4.2-hydrazinyl-6-methylpyrimidin-4(3H)-one 5.2-(2-(4-(dimethylamino)benzylidene)hydrazinyl)-6-methylpyrimidin-4(3H)-one.
Pyrimidine derivatives were identified by using spectroscopy (FTIR and UV-Visible) and by measuring their melting points. Part two: This part involved the affectivence of the synthesized pyrimidine derivatives on different cell lines in vitro, using two cell lines (HepG2 and MCF7), and by using Neutral red and MTT assays respectively, different concentrations of pyrimidine derivatives were prepared. The results showed an inhibitory effect on the growth of the two cell lines, 600µg/ml concentration showedthe best concentration for the best inhibition (IR) and gave the lowest percentage growth (Cell Viable) on two cell lines, also regression showed a significant negative relationship between pyrimidine derivatives and the cell lines growth, high concentration showed significant effect on cell lines growth.