Diabetes is a group of disorders that produce elevated levels of blood glucose. The two main forms of diabetes are type 1and type 2. The cause of diabetes was associated with some endocrine hormones and obesity. This study was focused on diabetes type 2 and its effects on thyroid hormones (TSH, T3, T4), prolactin and cortisol and with body mass index. To achieve this aim 68 diabetic patients type 2 with ages of 35-70 years and 34 healthy people with ages of 35-70 years (control group) were enrolled. These hormones (TSH, T3, T4, PRL and Cortisol) were estimated by an enzyme immunoassay method with final fluorescent detection (MiniVidas). The results demonstrated significant increase of T4 (p<0.05), Cortisol (p<0.05) levels, and significant decrease of TSH (p<0.05) levels. A non significant increase was shown in prolactin (p>0.05) levels, and non significant decrease in T3 (p>0.05) levels. Obesity is the most important modifiable risk factor in the pathogenesis of type 2 diabetes, however, racial factors seem to be important in the relationship between body mass index and glucose in patients with diabetes type 2. The linear regression analysis revealed significant negative correlation (r=-0.310, p<0.01) in cortisol levels with BMI, and significant positive correlation in fasting serum glucose (r=0.293, p<0.05) and TSH (r=0.275, p<0.05) levels with BMI in patients with diabetes type 2. The linear regression analysis revealed significant negative correlation (r=-0.249, p<0.05) in cortisol levels with fasting serum glucose, and positive correlation in TSH (r=0.258, p<0.05) levels with fasting serum glucose in patients with diabetes type 2. The linear regression analysis revealed significant negative correlation (r=-0.469, p<0.05) in T4 levels with Cortisol levels in patients with diabetes type 2.