This study was aimed to investigate the association between thyroid disorders and Helicobacter pylori infection in 122 patients (100 females and 22 males ) referred to the Specialized Center for Endocrinology and Diabetes at Al –kindy Hospital in Baghdad . For comparison, 60 healthy individuals (31 females and 29 males),who had no thyroid disorders, were also included in the study. Blood samples were collected from both patients and the healthy individuals. Enzyme Linked Fluorescent Assay (ELFA) technique through using Vitek Immuno Diagnostic Assay System (VIDAS) was applied to measure levels of the thyroid hormones (tri-iodothyronine T3, tetra-iodothyroxine T4) and thyroid stimulating hormone (TSH). From the results obtained, patients were classified into three groups: 40 were considered as belonging to the controlled * group ( 26 females and 14 males), 57 to the hypothyroidism group (52 females and 5 males) and 25 belonged to hyperthyroidism group ( 22 females and 3 males) . Females constituted the vast majority of both healthy and thyroid disorders patients with percentages of (51.7%) and (81.96%), respectively. On the other hand, highest incidence rate of thyroid disorders were recorded in the age group of (30-39)yrs. 19.67% , followed by (40-49)yrs. with 24.59% and(50-59)yrs. with 18.03%. The results also declared that there was a correlation between increasing the cholesterol level and decreasing level of high density lipoprotein (HDL) . When concentration and presence of anti -Helicobacter pylori IgG antibodies in the human blood samples were detected and measured by Enzyme Linked Immuno Sorrbent Assay (ELISA) technique , the results showed high prevalence rates of H. pylori infection detected in the hypothyroidism patients (94.07%), while the lowest prevalence rates were recorded in the healthy individuals ( 66.7%). Statistical analysis of anti –Helicobacter pylori IgG antibodies distribution among both healthy and thyroid patients showed that highly significant differences were found between thyroid disorders patients groups. Such results ensure the existence of the relationship between anti-H. pylori IgG antibodies and thyroid disorders occurrence.