Effect of Magnetic Field on Growth and Biochemical Indices of Some Fungi

number: 
3004
English
Degree: 
Imprint: 
Biotechnology
Author: 
Zainab Abbas Ali
Supervisor: 
Dr. Abdul-Ghani I. Yahya
Dr. Abdul-Wahid Sh. Jabir
year: 
2013
Abstract:

The effect of static magnetic field on the growth and biochemical indices of five fungal species were studied (Aspergillusniger,Alternariaalternate, Fusariumoxysporum,Penicilliumchrysogenium and Rhizopusoryzae). Exposing the above species to the northern pole, southern pole and both poles and their influences were compared with the control treatment (without magnetic field energy). The static magnetic field of 10 gauss was subjected to the above fungal species separatly for seven days at 28 °C, The effect of static magnetic field energy on the growth of fungal species on two solid media (SabouraudDextrose Agar (SDA) and Potato Dextrose Agar (PDA)) was classified as stimulatory, inhibitory and no observable effect on growth of fungal species. The effects of magnetic field poles (southern, northern and both) inhibited the growth ofFusariumoxysporum, while the magnetic field poles stimulated the growth of fungal species (Aspergillusniger,AlternariaalternataandPenicilliumchrysogenium) and had no observable effect of southern pole and both poles on growth of Rhizopusoryzae, while the northern pole inhibited the growth of R. oryzaecompared with control groupby measuring the growth diameter (cm), The effects of magnetic field poles on mycelia growth of the mentioned fungal species in liquid medium (Yeast Extract Sucrose(YES) broth) was determined by the dry weight measurement (g/100cm3) every 24 hr for 4 days, the content of flask was filtered through Whattman No.1fiterpaperto separatethe mycelial mat and culture filterate,and the fungi were effected by northern pole, southern pole only, The magnetic field poles were inhibited the growth of three species (A.alternata, F.oxysporumand R.oryzae) and increased in the growth of A.nigerand P. chrysogenium as compared with controls at the same condition. The effects of magnetic field poles on the biochemical indices of the fungal species were performed by filtering the growth and measuring the enzymes activity, protein and chitin concentration in the filterate, The fungal species were influenced by magnetic field energy for 3 days at 28°C, pH(6.5) showed increased in the activities of amylase and protease due to northern pole and decreasedwhen treated with southern pole however it was higher than the control treatment under optimum conditionat significant difference (at the .05 level),The northern pole increased amylase activity (U/ml) in the culture filterate of P. chrysogenum (0.246 U/ml) higher than other mentioned species,A. niger, F. oxysporum, R. oryzae and A. alternata (0.172, 0.146, 0.116, 0.105)U/ml respectively.The northern pole increased protease activity (U/ml) in the culture filterate of P. chrysogenum (0.081 U/ml) higher than other mentioned species,A. niger, A. alternata , R. oryzae and F. oxysporum (0.08, 0.074, 0.056,0.054)U/ml respectively, while protein concentration increase under the effect of magnetic field poles of 5 fungal species. The chitin concentration in liquid medium showed a decrease in chitin concentration (a polymer of N-acetylglucosamine) of (A.alternata, F.oxysporum and R.oryzae) and increased in the concentration of chitin of (A.nigerand P.chrysogenum) in treatments of magnetic field compared with controls (without treatment) at the same condition.