Characterization of new cross linked polymer as potential stationary phase for HPLC separation

A cross linked polymer was synthesized by condensation reaction between polyacrylic acid with succinic acid dihydrazide in the presence of phosphorus oxychloride. The products were characterized by FTIR. The resulted polymer has high rigidity and easily grinded, with high stability. This polymer was used as
stationary phase for HPLC. This stationary phase has been packed into stainless steel column. The chromatographic performance of the newly packed column was characterized. The number of theoretical plates (N), high equivalent to theoretical plate (H), capacity factor(K)and selectivity factor(a) were measured by analyzing different analytes on the new packed column using different mobile phase compositions and flow rates. Some of polyaromatic compounds sucli as phenanthrene ,acenaphthylene, anthracene and naphthalene were separated with this column either using mixture of (40%:60%), hexane and isopropanol or 100% isopropanol, as mobile phases, with flow rate Iml/min with UV detection at 254 nm. Furthermore some amino acids (tyrosine , Tryptophane, Phenylalanine, and Histidine ) were also separated using this column with a mixture of phosphate buffer at pli 6 in 10% acetonitrile or5% methanol at pH6.5 as mobile phase with flow rate of Iml/min with UV detection at 254 nm and RJ detector. Calibration curves for all the analyzed compounds were linear from their detection limits to at least 25 ppm with correlation coefficient range from 0.9995 to 0.9999. The detection limits were ranged from 0.05 to 0.1 ppm at signal to noise ratio of three or more for most analyzed compounds.