This study investigates the changes in some biochemical parameters such as total protein, calcium, aluminum, iron, and copper in the serum of chronic renal failure patients who were maintained on hemodialysis at Al-Yarmouk Teaching Hospital and Al-Karama Teaching Hospital. The blood of fifty patients (38 males and 12 females) were analyzed and compared with 30 healthy control subjects (19 males and 11 females). The blood samples were obtained before and after dialysis. The overall mean concentration of serum calcium, aluminum, and copper were found significantly higher than that of the controls (PO.001) while the mean concentration
of serum total protein and iron were found significantly lower than that of the control subjects (PO.05 and PO.001 respectively). All the above analytes have shown significant changes after dialysis except copper, however total protein, calcium, and aluminum increase (PO.001) and iron increase after dialysis (PO.001). To study the effect of duration on above analytes, there was a significant effect of duration on aluminum and iron concentrations (PO.001) and on copper in pre and post dialysis (PO.05). While other analytes had not affected by duration. On the other hand there was no significant effect of patient age on above analytes except copper (PO.05).