Specimens from both sexes and different ages were collected from Medical University Hospital of Al-Nahrien College of medicine, Al-Yarmouq Hospital, Medical Hospital and Al-Karama Hospital out of (256) expected to have urinary tract infection , (188) samples showed positive result in culture. From the positive result (56) sample were identified as Proteus . Isolation percentage of Proteus in samples taken from males was more than in females , as 71.4% and 28.6% respectively. P. mirabilis was the most common pathogen of-UTI with isolation percentage 80.35% as compared to that of P. vulgaris which was 19.6%. Susceptibility tests toward twenty three antibiotics were carried out . Results shows that gentamicin, amikacin and ciprofloxacin were the most effective antibiotics and their resistance percentage were 17.7% , 15.5% and 8% respectively . While ampicillin , climdamycin and rifampicin were not effective and their resistance percentage were 100% , 100% and 94% respectively. Eight isolates were selected depending on the result of antibiotic sensitivity tests as showed multi-antibiotic resistance and tested to specify their minimum inhibitory resistance and tested to specify their minimum inhibitory concentration for amoxicillin, cefatoxime, amikacin, gentamicin, nalidixic acid, ciprofloxacin, erythromycin and rifampicin. Ciprofloxacin , Gentamicin and Amikacin were found to have the lowest MIC as compared with other antibiotics.