Biotechnology - Sciene

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Fifty different soil samples (contaminated and uncontaminated with hydrocarbons compounds) were collected from different places in Iraq. Twenty five isolates showed the ability to  utilize methanol as a sole source of carbon and energy. These isolates were repeatedly tested in the defined medium supplemented with methanol to ensure methanol utilizing ability, then only 12 isolates that appeared, really have  the ability to utilize methanol and were named as (HK1,HK2,……,HK12), it was found that these isolates were varied in their growth density in utilizing methanol.