Extraction and Purification of Asparaginase enzyme from Pisum sativum plant and studying their cytotoxicity against L20B tumor cell line
Plant samples of Pisum sativum were collected from crop fields in the Collage of Agriculture/ University of Baghdad and were classified as Pisum sativum subspp. Jof according to their morphological characteristics. Activity of asparaginase was detected in seeds, stems and leaves extracts. Results showed that maximum asparaginase activity was detected in seeds extracts which was 30.0 U/mg in comparison with 26.4 and 16.1 U/mg in extracts of leaves and stems respectively.