M.Sc.

Extraction and Purification of Asparaginase enzyme from Pisum sativum plant and studying their cytotoxicity against L20B tumor cell line

 Plant samples of Pisum sativum were collected from crop fields in the Collage of Agriculture/ University of Baghdad and were classified as Pisum sativum subspp. Jof according to their morphological characteristics. Activity of asparaginase was detected in seeds, stems and leaves extracts. Results showed that maximum asparaginase activity was detected in seeds extracts which was 30.0 U/mg in comparison with 26.4 and 16.1 U/mg in extracts of leaves and stems respectively.

English

The Potential of Some Locally Grown Plants to Genetic Transformation with Cytochrome p450 and Their Use in Phytoremediation

To increase phytoremediation potential, Arabidopsis thaliana and Sesbania grandiflora were genetically engineered with the rabbit cytochrome P450 enzyme. The P450 2E1 enzyme controls the ratelimiting step in the metabolism of multiple environmental pollutants, including Trichloroethylene (TCE), Dichlorodiphenyltrichloroethane (DDT). The percentage of transformation increased at the less diluted cultures of Agrobacterium tumefaciens. Similarly, when bacterial concentration decreased, the chance of infected explants increased too and the concentration of A.

English

Treatment of Salmonella typhimurium with Probiotics to Evaluate it Histopathological and Immunological Effects on Mice

This study was aimed to investigate the protective effect of some probiotic microorganisms in mice infected with Salmonella typhimurium. A total of (119) samples including food, stool, and vaginal swabs were collected from various regions of Baghdad city. Lactobacillus spp. and Saccharomyces spp. were isolated from the samples to be used as probiotics. Twenty isolates of Lactobacillus spp., 17 of Saccharomyces cerevisiae and one isolate of Saccharomyces boulardii were obtained from the samples.

English