College of Science

 Six to eight weeks old white mice sharing the same mother and father were injected intraperitoneally with different dosages 0.1, 0.2 and 0.5mg/kg body weight (bw) of the chemotherapeutic antitumor drug Mitomycin C (MMC). The effect of the drug on mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) was investigated by molecular techniques and cytogenetic analyses were performed on spleen and bone marrow cells.

   The aim of this study was to initiate callus from black seed Nigella sativa to extract thymol substance and then study its potency to inhibit the  cytogenetic effects of mitomycin-C (MMC) on laboratory mice (in vivo).  Establishment of  Nigella sativa (black seed) seedlings in tissue culture was achieved in our laboratory by using Murashige and Skoog medium, (1962) (MS) and optimization of cultural medium using a combination of  2,4-D and Kin concentrations The seeds were cultured in sterilized distilled water (after their steriliza

         The present study was carried out to shed light on the chemical analysis of Glycyrrhiza glabra for different active compounds, and on the cytogenetic effects of methotrexate(MTX) and aqueous extract of G. glabra in laboratory mice  (in vivo) and human blood lymphocytes (in vitro). The study also aimed to investigate the role of G.