In this study 227 urine samples were collected from patients suffering from symptoms referred as urinary tract infection. The urine samples were obtained from different hospitals in Baghdad during the period from 1 / 12 / 2006 to 15 / 3 / 2007. 180 (64.98%) urine samples gave positive bacterial growth on MacConkey agar and / or blood agar. The obtained positive cultures showed that 34 (18.89%) were Gram positive bacteria and 146 (81.11%) samples were Gram negative. The Gram negative samples revealed that 98 (67.12%) bacterial isolates were identified as Escherichia coli and 48 bacterial isolates were identified as other species of Gram negative bacteria, 5 (3.4%) cultures were identified as Enterobacter sp., 11 (7.53%) isolates as Klebsiella sp., 15 (10.27%) isolates as Pseudomonas sp., and 17 (11.64%) isolates as Proteus sp. The percentage of E. coli isolates in samples taken from females was 60.56%, while the percentage of E. coli isolates which were identified in male samples was 39.44%. In this study we isolate seven bacteriophages from sewage water at different sites in Baghdad city. The isolates of phage were further purified, propagated and maintained for further tests to ascertain their ability to lyse various E. coli isolates that were obtained from UTI patients. In this study the lytic effect of seven bacteriophages was ascertained on 20 E. coli isolates. The results revealed 18 different isolates, and variable lytic activities were observed in different E. coli isolates. Therapeutic effect of bacteriophage was studied in treatment of experimentally infected mice skins with E. coli isolate obtained in this study. The results revealed the therapeutic effect of bacteriophage isolate number (R6) against infection of mice wound with E. coli isolate number (E14).