Study the Effect of Loranthus europaeus Extract on Wound Healing Process

Loranthus europaeus is an important medicinal plant, which contains a lot of bioactive compounds. The dried plant fruits were extracted in 80% methanol by maceration. Chemical detection of crud plant extracts was performed. The total flavonoids were isolated, subjected to thin layer chromatography (TLC) using different mobile systems. The purified material was augmented by using high performance liquid
chromatography (HPLC).Inflammation was induced in experimental animals (rabbits) by subcutaneous injection of 2.5% formalin. The anti-inflammatory effect of the extract was evaluated in healing skin wounds in comparison with the synthetic pharmaceutical medication Piroxicalm Gel. Simple preparation was applied by mixing the crude compound with Vaseline and Glycerin. After healing of the wound, topathological study was necessary to support the results.Results indicated that, L. europaeus fruits were rich with flavonoids of the total flavonoids were estimated, the major components were rutin and lueteolin and trace of quareciten and kaempferol.The healing of the skin was clear by disappearance of odema and reduction in scar size, enhancement of fibroblast proliferation, angiogenesis, keratinization and epithelialization as compared with the control groups. L. europaeus extracts could be considered as one of the promising plants for the treated of skin wound. Flavonoids of the plant have the ability of suppression of acute inflammation induced by chemical substance and seemed to be the most active component for healing the wound.