Genetic Polymorphism in SCL22A12 Gene Associated with Gout in a Sample of Iraqi Patients

number: 
3750
English
Degree: 
Author: 
Atyaf Adnan Naif
Supervisor: 
Prof. Dr . Waleed Hameed Yousif
year: 
2016

The current study aimed to  investigate the molecular defect causing the manifestation of gouty arthritis ,  the relationship between kidney function and the gout and , study the relationship between white  blood cell count and gout in a sample of  Iraqi patients  .The  study included 35 patients suffering from gout during the period  from  January 2015 to June 2015 at Al- Karamah and Al-Yarmouk  teaching hospitals .20 healthy subjects  were selected to represent the control group. The mean ages of  the patients were 34 -63 years and the mean ages of  the control were 25 - 40 years The personal information's  for each patient was  obtained , including  name , age ,gender, weight , length , medications and family history of gout . Uric and creatinine tests were performed to study relationship between the disease and kidney function .White blood cells was counted to investigate the inflammation status .DNA extraction was performed from whole blood samples. After DNA quantification, the DNA samples were   used for polymerase chain reaction (PCR) amplification .Agarose gel electrophoresis of PCR product showed the presence of band with size of 300 bp, 400bp, 600bp, 253bp and 296bp. After amplification of SCL22A12 gene that lead to gout.