This research was conducted to study the biological activity of Fumaria officinalis plant extract on laboratory animals. The herb Fumaria officinalis is a wide growing plant, has a wide reputation among herb- experts in Iraq, it's a folk traditions Few photochemical investigation of this plant had been reported previously but non on the Iraqi plant, thus indicating that a research on this plant might be of value Chemical detection was done to identify the active compound present in it. Two kinds of extracts were prepared from plant powder of Fumaria officinalis, that are aqueous and ethanolic extract, these two different extract of Fumaria officinalis powdered plant were tested for their in-vitro antimicrobial activity . In a screening program from the Iraqi alkaloid containing plants and chemical investigation showed that Fumaria officinalis contain different active compounds, the main active compound is the alkaloid, it was found that this plant is rich in alkaloid. Microorganisms, tested in this study were; Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Trichophyton rubrum, Trichophyton entagrophytes, and Trichophyton tonsurans. In -vitro tests showed that the best extract of Fumaria officinalis with highest antibacterial and antifungal activity is the ethanolic extract at a different concentrate. In contrast in- vitro showed that aqueous extract of Fumaria officinalis has a great antihistaminic activity rather than ethanolic extract. Also in-vitro studies of the values of liver enzyme (GPT, GOT, ALP, and BILL), theses values was varies due to effect of ethanolic extract of Fumaria officinalis.