A total of (100) samples were collected from various clinical specimens including (Burns, Wound, Sputum, Ear and Operation Room) and belonging to patients of both sexes and of different ages hospitalized in Al - Wassity, Al - Karkh and Saddam Medical Hospital in Baghdad. Only 50 isolates were characterized as Pseudemanas aerugfnosa by using Morphological, Physical and Biochemical tests. Susceptibility of the isolates to various antibiotics was also tested using disk diffusion method. Ciprofloxacin was to be the most + effective antibiotic with resistance frequency of 38 % followed by Neomyein 60 % while the remainders were less effective. Twelve isolates were selected in accordance to their pattern of resistance as those showing multi - drug resistance and tested to specify their minimum inhibitory concentration for Ciprofloxacin, Gentamicin, Piperacillin and Cefotaxime. Ciprofloxacin was found having the lowest MIC as compared with others Adherence capability of the isolates was also tested in — accordance to epithelial cells isolated from Human Mouth Cavity. All isolates were capable of adhering to epithelial cells. However, isolates No. 7 possessed the The effect of Sub MIC (o.75, 0.5, 0.3 and 0.25 ug/ml) of Piperacillin, centamycin, Cefotaxime and Ciprofloxatin on adhesion capability of the isolates were also specified. Cefotaxime was uneffective as compared to Centamycin, Ciprofloxacin and Piperacillin as tested visually using light microscope. Pilin was isolated from culture of isolate No. 7 exposed to various concentrations of antibiotics at Sub MICs level Poly Acrylamide Cel Ekctrophoresis (PACE) profile of pilin indicate a variation among treatments. However, 0.75 ug/ml MIC of Ciprofloxacin show the highest activity as compared with others. Results of protein profile followed the same trend as that of adhesion experiment and that demonstrate the effect of pilin adhesion capability of the isolates. Sparse alginate contents were detected in the isolates selected for adhesion experiment which indicates that these isolates are piliated non mucoid. Inn viva study of antibiotics on infectivity and hence adhesion of P. aerug/nesa was also done using infant mouse model. The results indicate that 0.75 ug/ml of Ciprofloxacin was effective in reducing number of P. aerugfnasa which also causes a reduction in infection capability of the isolate and progress of the. disease. highest capacity with mean number of adhering bacteria of (13.5) as compared with others.
The effect of Sub MIC (o.75, 0.5, 0.3 and 0.25 ug/ml) of Piperacillin, Centamycin, Cefotaxime and Ciprofloxatin on adhesion capability of the isolates were also specified. Cefotaxime was uneffective as compared to Centamycin, Ciprofloxacin and Piperacillin as tested visually using light microscope. Pilin was isolated from culture of isolate No. 7 exposed to various concentrations of antibiotics at Sub MICs level Poly Acrylamide Cel Ekctrophoresis (PACE) profile of pilin indicate a variation among treatments. However, 0.75 ug/ml MIC of Ciprofloxacin show the highest activity as compared with others. Results of protein profile followed the same trend as that of adhesion experiment and that demonstrate the effect of pilin adhesion capability of the isolates. Sparse alginate contents were detected in the isolates selected for adhesion experiment which indicates that these isolates are piliated non mucoid. Inn viva study of antibiotics on infectivity and hence adhesion of P. aerug/nesa was also done using infant mouse model. The results indicate that 0.75 ug/ml of Ciprofloxacin was effective in reducing number of P. aerugfnasa which also causes a reduction in infection capability of the isolate and progress of the. disease.